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Aquasure 3000
TOGS 9000
AGACSS Software

TOGS 9000 and System Components

The TOGS 9000 is a single sample microprocessor controlled, isothermal spectronic device (ISD) with all of the benefits of the Aquasure PRO 3000 plus the ability to conduct quantitative analysis using Fluorometric, Colorimetric & Nephlometric Detection, and data storage and communications capability, even from remote areas, without a computer.

TOGS 9000 Detection Systems

The TOGS 9000 Features Three Detection Systems Operating Simultaneously during Incubation - all done automatically and non-intrusively.

Fluorometric Detection for E.coli

The fluorigenic enzyme substrate such as 4-methylumbelliferyl -β-D-glucuronide (MUG) when hydrolyzed by the GUD from e.coli produces the fluorescing compound 4-methylumberlliferone (4MU). The, e.coli present in the sample multiplies and interacts with the MUG present in the medium, during incubation which in turn releases the 4MU into the solution. This fluorogen when subjected to UV radiation at ~ 360 nm re-emits the energy absorbed in the form of light radiation (~ 450 nm). The emitted radiation thus has a different but longer wavelength than the absorbed radiation (the fluorescence effect). The emission is random and non-directional. Thus it can be measured at any angle. As the number of e.coli increases, the intensity of the fluorescence emission increases. Low levels of emission intensity are measured accurately and provide an early indication of the presence of e.coli in sample.

Colorimetric Detection for Total Coliform

The chromogenic enzyme substrate such as X-GAL is converted by the enzyme produced by coliforms into the indigo-blue chromophore. During the incubation period, coliforms present in the sample multiply and interact with the X-GAL present in the medium, which in turn releases the indigo dye into the solution. This chromophore when subjected to radiation at ~ 680 nm absorbs the energy. The unabsorbed (transmitted) radiation is measured using an appropriate photo detector. As the number of coliforms increases, the intensity of the transmitted radiation reaching the detector decreases. Low levels of absorption are measured accurately and provide an early indication of the presence of coliforms in sample.

Nephelometric Detection for Total Bacteria

Turbidity is used as a measure of the presence of total bacteria in water samples. During the course of the incubation the transparent growth medium progressively becomes more cloudy and opaque indicating the growth of bacteria in the sample. The increasing turbidity during incubation is monitored using an optical spectroscopic detection technique.

Similar to the colorimetric and fluorometric detection systems, simultaneous to incubation the turbidity produced by the active bacteria present in the fluid sample is measured, as the bacteria present in the sample multiplies and interacts with the nutrient substrate, which in turn produces suspended particles (turbidity) in the solution.

When subjected to incident radiation, these suspended particles scatter the light energy in all directions. The scattered radiation has the same wavelength as that of the incident radiation and is detected at any angle (Nephelometric). As the number of bacteria increases, the turbidity increases, and the intensity of the scattered radiation increases. Using a light source and the appropriate detector, low levels of scattered light intensity are accurately measured thus providing early indication of the presence of bacteria (total) in the sample.

This way total bacteria count can be determined in a water sample using a nutrient growth media. With the use of an X-GAL/MUG reagent, the turbidity produced during the early stages of the growth period is solely attributed to the presence of coliforms as no other bacteria interfere by producing turbidity. Therefore the turbidity measurement can also be used as confirmatory test for coliforms & e.coli measurement.

Unique Features of the TOGS System

  • Simultaneously incubates and monitors the sampling process;
  • Is non-intrusive and one step sampling process;
  • Provides full automation and speed without the need of complex robotics;
  • Provides real-time microbial growth information;
  • Provides the ability to remotely monitor & control tests potentially worldwide from a single location;
  • Configuration capability to provide automatic reporting to government regulators;
  • Programmable alarms with multiple paging and auto-call capabilities, provides unattended operation capability and reporting, even on weekends and holidays;
  • Location and product identifiers provide the ability with time and date stamp function enabled, to monitor and batch trends and other statistical information;
  • Provides the ability to transfer information direct from the TOGS 9000 in remote locations without the need of a computer, through cellular, satellite phone or land line communication;
  • AGACSS Remote User Software, provides the ability for field service personnel, to monitor, record and print information in real-time, even in remote locations (PC or mobile device required);
  • Enhanced system and data integrity with customer programmable multiple level permissions based information access (AGACSS network version only);
  • Built-in Auto Quality Control provides real-time drift correction, real-time blank measurement, auto-calibration and real-time and abort feature, with every test;
  • Cross confirmation is achieved through flashing LEDs on TOGS 9000 (P/A). In addition, turbidity results are confirmed through both colorimetric and fluorometric measurement;

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